Custom Multi-Wavelength LED Light Source

The 7+1 custom multi-wavelength fiber coupled LED light source was developed for Biophotonics lab at Advanced Technologies Center (ATC) at Sheba Medical Center, Tel-Hashomer Israel. This system is a custom version of Prizmatix FC5-LED multi-wavelength system. The FC2-3-5 fiber optic coupled LED system comprises UV, Violet, Blue, Green and RED LED at wavelengths chosen by the customer.

The specific system built for ATC comprises 7 fiber coupled LED sources at predefined wavelengths, each with separate driver LED driver and separate control. The control options allow manual power adjustment using a 10 turns potentiometer on the front panel, ON/OFF switch of each channels, TTL input for chopped operation or for computer control through A/D D/A card with digital outputs (DIO). On the picture one can see the first channel (from left) is connected to a fiber patch cord. The last channel (at right) is an electrical connector instead of optical connector. This additional channel provides driver and control with a connector for future optional system expansion by connection of Black-LED head - a fiber coupled LED light source at different wavelength.

This film shows the FC5-LED system used with a fiber optic bundle probe connected to a miniature fiber optic spectrometer: http://www.youtube.com/watch?v=qwMpxBgDFJU

UHP-MIC-LED-460 Compared to Mercury Lamp

Classical Mercury Lamps are gradually giving way to new illumination systems based on high-power LEDs. Prizmatix offers two series of microscope fluorescence illumination: the standard High Power MIC-LED series and the new Ultra High Power UHP-MIC-LED series. Prizmatix’s compact LED light source modules are an effective replacement of lasers and Hg lamps in many applications.

The above images were taken with an Olympus IX81 inverted microscope equipped with a Qimaging Retiga 1300R monochrome camera. Note that the exposure time with the Mercury lamp (right) is 5000 msec, while the exposure time with the UHP-Mic-LED-460 is 2500 msec (left).

Sample: Molecular Probes prepared slide #3 (kidney cells) stained with Alexafluor 488.

Images courtesy of G. Sobocinski, University of Michigan.

As demonstrated in the above images, the Prizmatix UHP-Mic-LED-460 is significantly brighter than 100W mercury lamps.

Key Features:

* Compatible with Prizmatix modular Microscope LED Light Source products range

* TTL external triggering

* Excellent for fluorescence excitation

* LED spectrum can be narrowed by optional band pass filter installed inside the LED head

* Stable, precisely adjustable power

* Long life (no lamp or laser tube replacement required)

* Speckle free

* Rapid warm up time

Applications:

* Fluorescence microscopy Auramine, di-8-ANEPPS, GFP, Quantum Dots

* Whole body imaging of small animals in-vivo

* Photo activation (PA)

* Photostimulation of Channelrhodopsin ChR1, ChR2

* Fluorescence recovery after photo bleach (FRAP)

* Fluorescence in situ hybridization (FISH)

* Bio analysis

* Machine Vision